Our laboratory is dedicated to developing a high-throughput cryo-electron tomography (cryo-ET) pipeline for high-resolution structure determination of molecular machines in cells. Our state-of-the-art imaging system has been utilized to gain molecular insights into fundamental biochemical processes: bacterial motility, chemotactic signaling, protein secretion, and DNA translocation.
Electron Tomography at ut houston
Interested candidates should have a Ph.D. in microbiology, biophysics or a related field. Please contact Dr. Liu along with CV, statement of research interests, and contact information for three references.
Cryo-Electron Tomography (cryo-ET) is one of the most exciting technologies to determine 3-D architecture of molecular machines in situ at molecular level. Our high-throughput cryo-ET pipeline provides unparalleled opportunities for visualizing the molecular machines in cells.
1. Phage T7 Walks across bacterial surface.
2. Bacterial secretion system revealed in intact cell.
3. No grant writing, no funding support.
We have a 300 kV Liquid Nitrogen cooled field-emission gun cryo-electron microscope (FEI Polara) and Tietz F415SP 4k x 4k CCD camera at UT Houston. This cutting-edge cryo-EM facility supports multiple NIH funded projects. In 2013, we got a Shared Instrumentation Grant from NIH and replaced our CCD camera with a new generation direct electron detector (Gatan K2 Summit). Together with advanced tomography packages (SerialEM, IMOD, TOMO3D and Protomo) implemented, our system is now able to achieve higher throughput and resolution.
The LIU lab
We are very grateful that our research projects are supported by NIH, NIAID, NIGMS, and Welch Foundation.
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